Anti-Phospholipase C beta 3 (phospho S1105) antibody (ab52206)

Overview

  • Product nameAnti-Phospholipase C beta 3 (phospho S1105) antibody
    See all Phospholipase C beta 3 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase C beta 3 (phospho S1105)
  • Specificityab52206 detects endogenous levels of Phospholipase C beta 3 only when phosphorylated at serine 1105.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Phospholipase C beta 3 around the phosphorylation site of serine 1105 (N-N-SP-I-S).

  • Positive control
    • Human brain tissue; extracts from A431 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-3 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta-3 antibody
    • Phospholipase C beta 3 antibody
    • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
    • Phospholipase C-beta-3 antibody
    • PLC beta 3 antibody
    • PLC-beta-3 antibody
    • Plcb3 antibody
    • PLCB3_HUMAN antibody
    see all

Anti-Phospholipase C beta 3 (phospho S1105) antibody images

  • This image shows human brain tissue stained with ab52206 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
  • All lanes : Anti-Phospholipase C beta 3 (phospho S1105) antibody (ab52206) at 1/500 dilution

    Lane 1 : extracts from A431 cells with no immunising peptide
    Lane 2 : extracts from A431 cells with immunising peptide


    Predicted band size : 139 kDa
    Observed band size : 139 kDa

References for Anti-Phospholipase C beta 3 (phospho S1105) antibody (ab52206)

ab52206 has not yet been referenced specifically in any publications.

Product Wall

The lab recommends to dilute the antibodies in TBST with Tween.



I hope this answers your question. Please contact us if you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"