Anti-Phospholipase c eta 1 antibody (ab150838)

Overview

  • Product name
    Anti-Phospholipase c eta 1 antibody
    See all Phospholipase c eta 1 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase c eta 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids of Human Phospholipase c eta 1.

  • Positive control
    • Human cerebral cortex tissue; U2 OS cells.
  • General notes
    The antibody solution should be gently mixed before use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. •Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes.
  • Tissue specificity
    Expressed in brain and to a lower extent in lung. In brain, it is found in cerebrum, cerebellum and spinal cord.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 2 EF-hand domains.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1-phosphatidylinositol 4 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase eta-1 antibody
    • 5-bisphosphate phosphodiesterase eta-1 antibody
    • DKFZp434C1372 antibody
    • KIAA1069 antibody
    • MGC117152 antibody
    • Phosphoinositide phospholipase C-eta-1 antibody
    • Phospholipase C-eta-1 antibody
    • Phospholipase C-like protein 3 antibody
    • PLC-eta-1 antibody
    • PLC-L3 antibody
    • PLCeta1 antibody
    • Plch1 antibody
    • PLCH1_HUMAN antibody
    • PLCL3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Phospholipase c eta 1 with ab150838 antibody at 1/200 dilution.
  • Immunofluorescent analysis of PFA/Triton X-100-fixed U 2 OS cells labeling Phospholipase c eta 1 with ab150838 at 4 µg/ml.

References

ab150838 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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