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Recombinant full length protein corresponding to Human Phospholipase C gamma 1.
Database link: P19174
Our Abpromise guarantee covers the use of ab16955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 149 kDa.|
|IP||Use a concentration of 1 µg/ml.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab16955 observed at 160 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab16955 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab16955 at a dilution of 1/2000 and ab181602 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.