The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
Use at an assay dependent concentration.
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ubiquitinated by CBLB in activated T-cells.
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Immunohistochemistry (Paraffin-embedded sections) - Phospholipase C gamma 1 antibody (ab52200)
Paraffin-embedded human breast carcinoma tissue stained for Phospholipase C gamma 1, using ab52200 (1/100). The right hand panel represents a negative control where ab52200 was pre-incubated with the immunizing (blocking) peptide.
Western blot - Phospholipase C gamma 1 antibody (ab52200)
All lanes : Anti-Phospholipase C gamma 1 antibody (ab52200) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated with EGF (200ng/ml, 30min). Lane 2 : Extracts from COS7 cells treated with EGF (200ng/ml, 30min). For this panel, ab52200 was pre-icubated with the immunizing (blocking) peptide.
Predicted band size: 149 kDa Observed band size: 149 kDa