Anti-Phospholipase C gamma 1 antibody (ab52200)

Overview

  • Product name
    Anti-Phospholipase C gamma 1 antibody
    See all Phospholipase C gamma 1 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase C gamma 1
  • Host species
    Rabbit
  • Specificity
    This antibody detects endogenous levels of total Phospholipase C gamma 1 protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide designed around the phosphorylation site of tyrosine 771 of Human Phospholipase C gamma 1 .

  • Positive control
    • Human breast carcinoma tissue (IHC-P).

Properties

Applications

Our Abpromise guarantee covers the use of ab52200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • Function
    Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • Post-translational
    modifications
    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • Cellular localization
    Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • Phosphatidylinositol phospholipase C antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • Plcg1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    see all

Images

  • Paraffin-embedded human breast carcinoma tissue stained for Phospholipase C gamma 1, using ab52200 (1/100). The right hand panel represents a negative control where ab52200 was pre-incubated with the immunizing (blocking) peptide.
  • All lanes : Anti-Phospholipase C gamma 1 antibody (ab52200) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated
    with EGF (200ng/ml, 30min).
    Lane 2 : Extracts from COS7 cells treated
    with EGF (200ng/ml, 30min). For this panel, ab52200 was pre-icubated with the immunizing (blocking) peptide.

    Predicted band size: 149 kDa
    Observed band size: 149 kDa

References

ab52200 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab52200.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up