Validated using a knockout cell line
Recombinant
RabMAb

Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] (ab76155)

Overview

  • Product name
    Anti-Phospholipase C gamma 1 antibody [EP1898-7Y]
    See all Phospholipase C gamma 1 primary antibodies
  • Description
    Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Phospholipase C gamma 1.

  • Positive control
    • Jurkat cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-Phospholipase C gamma 1 antibody (HRP) [EP1898-7Y] (ab195861)

Properties

Applications

Our Abpromise guarantee covers the use of ab76155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
IP 1/50.
ICC 1/250 - 1/500.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
    • Sequence similarities
      Contains 1 C2 domain.
      Contains 1 EF-hand domain.
      Contains 2 PH domains.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • Domain
      The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
    • Post-translational
      modifications
      The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
      Ubiquitinated by CBLB in activated T-cells.
    • Cellular localization
      Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
      • Inositoltrisphosphohydrolase antibody
      • Monophosphatidylinositol phosphodiesterase antibody
      • NCKAP3 antibody
      • Phosphatidylinositol phospholipase C antibody
      • Phosphoinositidase C antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-1 antibody
      • Phospholipase C 148 antibody
      • Phospholipase C gamma 1 antibody
      • Phospholipase C-gamma-1 antibody
      • Phospholipase C-II antibody
      • PLC gamma 1 antibody
      • PLC II antibody
      • PLC-148 antibody
      • PLC-gamma-1 antibody
      • PLC-II antibody
      • PLC1 antibody
      • PLC148 antibody
      • Plcg1 antibody
      • PLCG1_HUMAN antibody
      • PLCgamma1 antibody
      see all

    Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] images



    • Predicted band size : 149 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: Mouse brain tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab76155 was shown to specifically react with Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry analysis of Jurkat ( Human acute T cell leukemia) labeling Phospholipase C gamma 1 with purified ab76155 at 1/200 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde  and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] (ab76155) at 1/10000 dilution + Jurkat cell lysates at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 149 kDa
      Observed band size : 149 kDa

    References for Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] (ab76155)

    ab76155 has not yet been referenced specifically in any publications.

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