The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
1/250 - 1/500.
Application notesIs unsuitable for Flow Cyt or IHC-P.
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Post-translational modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] images
Western blot - Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] (ab76155)
Predicted band size : 149 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg) Lane 3: HepG2 cell lysate (20 µg) Lane 4: Mouse brain tissue lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76155 was shown to specifically react with Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Phospholipase C gamma 1 antibody [EP1898-7Y] (ab76155)
Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] (ab76155) at 1/10000 dilution + Jurkat cell lysates at 10 µg
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 149 kDa Observed band size : 149 kDa
References for Anti-Phospholipase C gamma 1 antibody [EP1898-7Y] (ab76155)
has not yet been referenced specifically in any publications.
Publishing research using ab76155? Please let us know so that we can cite the reference in this datasheet.
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