1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for ICC,IHC-P or IP.
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Post-translational modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Anti-Phospholipase C gamma 1 antibody [EPR5358] images
Western blot - Anti-Phospholipase C gamma 1 antibody [EPR5358] (ab109501)
Predicted band size : 149 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg) Lane 3: HepG2 cell lysate (20 µg) Lane 4: Mouse brain tissue lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109501 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab109501 at a dilution of 1/1000 and ab8245 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Phospholipase C gamma 1 antibody [EPR5358] (ab109501)
All lanes : Anti-Phospholipase C gamma 1 antibody [EPR5358] (ab109501) at 1/1000 dilution
Lane 1 : HepG2 cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 149 kDa
Flow Cytometry - Phospholipase C gamma 1 antibody [EPR5358] (ab109501)
Flow cytometric analysis of permeabilized Jurkat cells using ab109501 at 1/10 dilution (red)or a rabbit IgG (negative) (green).
References for Anti-Phospholipase C gamma 1 antibody [EPR5358] (ab109501)
has not yet been referenced specifically in any publications.
Publishing research using ab109501? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"