The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Post-translational modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Anti-Phospholipase C gamma 1 (phospho Y1253) antibody images
Western blot - Phospholipase C gamma 1 (phospho Y1253) antibody (ab73993)
All lanes : Anti-Phospholipase C gamma 1 (phospho Y1253) antibody (ab73993) at 1/500 dilution
Lane 1 : extracts from LOVO cells Lane 2 : extracts from 293 cells Lane 3 : extracts from LOVO cells with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 149 kDa Observed band size : 149 kDa Additional bands at : 125 kDa,72 kDa,95 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Phospholipase C gamma 1 (phospho Y1253) antibody (ab73993)
This product has been referenced in:
Shi B et al. Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor. Neoplasia15:669-83 (2013).
Read more (PubMed: 23730215) »