Anti-Phospholipase C gamma 1 (phospho Y1253) antibody (ab73993)

Overview

  • Product nameAnti-Phospholipase C gamma 1 (phospho Y1253) antibody
    See all Phospholipase C gamma 1 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase C gamma 1 (phospho Y1253)
  • Specificityab73993 detects Phospholipase C gamma 1 only when phosphorylated at tyrosine 1253.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide derived from human Phospholipase C gamma 1 around the phosphorylation site of tyrosine 1253 (S-R-YP-Q-Q).

  • Positive control
    • heat shocked extracts from LOVO cells and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab73993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
ELISA 1/20000.

Target

  • FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • Post-translational
    modifications
    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • Phosphatidylinositol phospholipase C antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • Plcg1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    see all

Anti-Phospholipase C gamma 1 (phospho Y1253) antibody images

  • All lanes : Anti-Phospholipase C gamma 1 (phospho Y1253) antibody (ab73993) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from 293 cells
    Lane 3 : extracts from LOVO cells with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 149 kDa
    Observed band size : 149 kDa
    Additional bands at : 125 kDa,72 kDa,95 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Phospholipase C gamma 1 (phospho Y1253) antibody (ab73993)

This product has been referenced in:
  • Shi B  et al. Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor. Neoplasia 15:669-83 (2013). Read more (PubMed: 23730215) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"