Anti-Phospholipase C gamma 1 (phospho Y1253) antibody [EP1502Y] (ab81284)


  • Product nameAnti-Phospholipase C gamma 1 (phospho Y1253) antibody [EP1502Y]
    See all Phospholipase C gamma 1 primary antibodies
  • Description
    Rabbit monoclonal [EP1502Y] to Phospholipase C gamma 1 (phospho Y1253)
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding tyrosine 1245 of human Phospholipase C gamma 1.

  • Positive control
    • Jurkat + Pervanadate cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Our Abpromise guarantee covers the use of ab81284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 149 kDa.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 EF-hand domain.
      Contains 2 PH domains.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
    • Post-translational
      The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
      Ubiquitinated by CBLB in activated T-cells.
    • Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
      • Inositoltrisphosphohydrolase antibody
      • Monophosphatidylinositol phosphodiesterase antibody
      • NCKAP3 antibody
      • Phosphatidylinositol phospholipase C antibody
      • Phosphoinositidase C antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-1 antibody
      • Phospholipase C 148 antibody
      • Phospholipase C gamma 1 antibody
      • Phospholipase C-gamma-1 antibody
      • Phospholipase C-II antibody
      • PLC gamma 1 antibody
      • PLC II antibody
      • PLC-148 antibody
      • PLC-gamma-1 antibody
      • PLC-II antibody
      • PLC1 antibody
      • PLC148 antibody
      • Plcg1 antibody
      • PLCG1_HUMAN antibody
      • PLCgamma1 antibody
      see all

    Anti-Phospholipase C gamma 1 (phospho Y1253) antibody [EP1502Y] images

    • All lanes : Anti-Phospholipase C gamma 1 (phospho Y1253) antibody [EP1502Y] (ab81284) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat + Pervanadate cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 149 kDa
      Observed band size : 149 kDa

    References for Anti-Phospholipase C gamma 1 (phospho Y1253) antibody [EP1502Y] (ab81284)

    ab81284 has not yet been referenced specifically in any publications.

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