Anti-Phospholipase C gamma 1 (phospho Y783) antibody (ab53125)

Overview

  • Product nameAnti-Phospholipase C gamma 1 (phospho Y783) antibody
    See all Phospholipase C gamma 1 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase C gamma 1 (phospho Y783)
  • Specificityab53125 detects endogenous levels of Phospholipase C gamma 1 only when phosphorylated at tyrosine 783.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic phosphopeptide derived from human Phospholipase C gamma 1 around the phosphorylation site of tyrosine 783 (G-F-YP-V-E).

  • Positive control
    • Human breast carcinoma tissue and cos7 cell extracts treated with EGF (200ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab53125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • Post-translational
    modifications
    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • Phosphatidylinositol phospholipase C antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • Plcg1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    see all

Anti-Phospholipase C gamma 1 (phospho Y783) antibody images

  • All lanes : Anti-Phospholipase C gamma 1 (phospho Y783) antibody (ab53125) at 1/300 dilution

    Lane 1 : cos7 cell extract treated with EGF (200ng/ml, 30mins)
    Lane 2 : cos7 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide


    Predicted band size : 149 kDa
  • ab53125 at 1/50 dilution staining Phospholipase C gamma 1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References for Anti-Phospholipase C gamma 1 (phospho Y783) antibody (ab53125)

This product has been referenced in:
  • Xu R  et al. Oxidative stress mediates the disruption of airway epithelial tight junctions through a TRPM2-PLC?1-PKCa signaling pathway. Int J Mol Sci 14:9475-86 (2013). WB ; Human . Read more (PubMed: 23629676) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"