Phospholipase D Activity Assay Kit (Colorimetric) (ab183306) provides a simple and sensitive method of measuring phospholipase D (PLD) activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from phosphatidylcholine. The free choline is then oxidized by the PLD enzyme mix to generate an intermediate which reacts with the PLD Probe to generate color that is detected at OD= 570 nm. This high-throughput adaptable assay kit can detect as little as 1.0 mU/mL PLD activity in cells and tissues.
Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders.
Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.