Phospholipase D Activity Assay Kit (Colorimetric) (ab183306)

Overview

  • Product name
    Phospholipase D Activity Assay Kit (Colorimetric)
    See all Phospholipase D kits
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Enzyme activity
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    Phospholipase D Activity Assay Kit (Colorimetric) (ab183306) provides a simple and sensitive method of measuring phospholipase D (PLD) activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from phosphatidylcholine. The free choline is then oxidized by the PLD enzyme mix to generate an intermediate which reacts with the PLD Probe to generate color that is detected at OD=  570 nm. This high-throughput adaptable assay kit can detect as little as 1.0 mU/mL PLD activity in cells and tissues.

  • Notes

    Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Choline Standard (Lyophilized) 1 vial
    PLD Assay Buffer 1 x 25ml
    PLD Enzyme Mix (Lyophilized) 1 vial
    PLD Positive Control (Lyophilized) 1 vial
    PLD Probe (in DMSO) 1 x 200µl
    PLD Substrate 1 x 100µl
  • Function
    Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
  • Tissue specificity
    Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
  • Sequence similarities
    Belongs to the phospholipase D family.
    Contains 1 PH domain.
    Contains 2 PLD phosphodiesterase domains.
    Contains 1 PX (phox homology) domain.
  • Cellular localization
    Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
  • Information by UniProt
  • Alternative names
    • Choline phosphatase 1
    • hPLD1
    • Phosphatidylcholine-hydrolyzing phospholipase D1
    • Phospholipase D1
    • PLD 1
    • PLD1
    • PLD1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab183306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Typical Choline Standard calibration curve.

  • PLD activity in liver lysate (5 µL) & Positive Control (1 µL).

Protocols

References

ab183306 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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