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Phospholipase D Activity Assay Kit (Colorimetric) (ab183306) provides a simple and sensitive method of measuring phospholipase D (PLD) activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from phosphatidylcholine. The free choline is then oxidized by the PLD enzyme mix to generate an intermediate which reacts with the PLD Probe to generate color that is detected at OD= 570 nm. This high-throughput adaptable assay kit can detect as little as 1.0 mU/mL PLD activity in cells and tissues.
Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders.
|Choline Standard (Lyophilized)||1 vial|
|PLD Assay Buffer||1 x 25ml|
|PLD Enzyme Mix (Lyophilized)||1 vial|
|PLD Positive Control (Lyophilized)||1 vial|
|PLD Probe (in DMSO)||1 x 200µl|
|PLD Substrate||1 x 100µl|
Our Abpromise guarantee covers the use of ab183306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab183306 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"