Anti-Phospholipase D1 antibody - Aminoterminal end (ab50695)

Overview

  • Product nameAnti-Phospholipase D1 antibody - Aminoterminal end
    See all Phospholipase D1 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase D1 - Aminoterminal end
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide located in the N-terminal region of human Phospholipase D1

Properties

Applications

Our Abpromise guarantee covers the use of ab50695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionImplicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
  • Tissue specificityExpressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
  • Sequence similaritiesBelongs to the phospholipase D family.
    Contains 1 PH domain.
    Contains 2 PLD phosphodiesterase domains.
    Contains 1 PX (phox homology) domain.
  • Cellular localizationCytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choline phosphatase 1 antibody
    • hPLD1 antibody
    • Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
    • Phospholipase D1 antibody
    • PLD 1 antibody
    • PLD1 antibody
    • PLD1_HUMAN antibody
    see all

References for Anti-Phospholipase D1 antibody - Aminoterminal end (ab50695)

This product has been referenced in:
  • Zhang ZR  et al. Anionic phospholipids differentially regulate the epithelial sodium channel (ENaC) by interacting with alpha, beta, and gamma ENaC subunits. Pflugers Arch 459:377-87 (2010). ICC/IF ; Xenopus laevis . Read more (PubMed: 19763606) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (murine embryonic fibroblasts)
Specification murine embryonic fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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Verified customer

Submitted Dec 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2-OS)
Specification U2-OS
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

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Verified customer

Submitted Jul 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"