Overview

  • Product nameAnti-Phospholipase D1 antibody [EP1506Y]
    See all Phospholipase D1 primary antibodies
  • Description
    Rabbit monoclonal [EP1506Y] to Phospholipase D1
  • Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N terminus of human Phospholipase D1.

  • Positive control
    • Hela cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab68150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionImplicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
    • Tissue specificityExpressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
    • Sequence similaritiesBelongs to the phospholipase D family.
      Contains 1 PH domain.
      Contains 2 PLD phosphodiesterase domains.
      Contains 1 PX (phox homology) domain.
    • Cellular localizationCytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Choline phosphatase 1 antibody
      • hPLD1 antibody
      • Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
      • Phospholipase D1 antibody
      • PLD 1 antibody
      • PLD1 antibody
      • PLD1_HUMAN antibody
      see all

    Anti-Phospholipase D1 antibody [EP1506Y] images

    • Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + Hela cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 120 kDa
      Observed band size : 120 kDa

    References for Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)

    This product has been referenced in:
    • Sulzmaier FJ  et al. PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D. Oncogene 31:3547-60 (2012). Read more (PubMed: 22105357) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"