Overview

  • Product name
    Anti-Phospholipase D1 antibody [EP1506Y]
    See all Phospholipase D1 primary antibodies
  • Description
    Rabbit monoclonal [EP1506Y] to Phospholipase D1
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N terminus of human Phospholipase D1.

  • Positive control
    • Hela cell lysate
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab68150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
    • Tissue specificity
      Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
    • Sequence similarities
      Belongs to the phospholipase D family.
      Contains 1 PH domain.
      Contains 2 PLD phosphodiesterase domains.
      Contains 1 PX (phox homology) domain.
    • Cellular localization
      Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Choline phosphatase 1 antibody
      • hPLD1 antibody
      • Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
      • Phospholipase D1 antibody
      • PLD 1 antibody
      • PLD1 antibody
      • PLD1_HUMAN antibody
      see all

    Images

    • Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + Hela cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 120 kDa
      Observed band size : 120 kDa

    References

    This product has been referenced in:
    • Sulzmaier FJ  et al. PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D. Oncogene 31:3547-60 (2012). Read more (PubMed: 22105357) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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