Anti-Phospholipase D2 (phospho Y169) antibody (ab78907)

Overview

  • Product name
    Anti-Phospholipase D2 (phospho Y169) antibody
    See all Phospholipase D2 primary antibodies
  • Description
    Rabbit polyclonal to Phospholipase D2 (phospho Y169)
  • Specificity
    ab78907 detects endogenous levels of Phospholipase D2 only when phosphorylated at tyrosine 169.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Phospholipase D2 around the phosphorylation site of tyrosine 169 (E-N-YP-L-N)

  • Positive control
    • IHC-P: human brain tissue WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)

Properties

Applications

Our Abpromise guarantee covers the use of ab78907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 106 kDa.
IHC-P 1/50 - 1/100.

Target

Images

  • All lanes : Anti-Phospholipase D2 (phospho Y169) antibody (ab78907) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
    Lane 2 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 106 kDa
    Observed band size : 94 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of Phospholipase D2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.

References

ab78907 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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