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Rabbit polyclonal to Phospholipase D2 (phospho Y169)
ab78907 detects endogenous levels of Phospholipase D2 only when phosphorylated at tyrosine 169.
Predicted to work with:
Synthetic phosphopeptide derived from human Phospholipase D2 around the phosphorylation site of tyrosine 169 (E-N-Y
IHC-P: human brain tissue
WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab78907 was purified by affinity chromatography using an epitope specific phosphopeptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 106 kDa.
1/50 - 1/100.
May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Belongs to the phospholipase D family.
Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.
Information by UniProt
Choline phosphatase 2 antibody
EC 188.8.131.52 antibody
hPLD 2 antibody
Western blot - Phospholipase D2 (phospho Y169) antibody (ab78907)
All lanes :
Anti-Phospholipase D2 (phospho Y169) antibody (ab78907) at 1/500 dilution
Lane 1 :
extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
Lane 2 :
extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size:
Observed band size:
94 kDa (
why is the actual band size different from the predicted?
Additional bands at:
140 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Phospholipase D2 (phospho Y169) antibody (ab78907)
Immunohistochemical analysis of Phospholipase D2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.
has not yet been referenced specifically in any publications.
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