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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab78907 was purified by affinity chromatography using an epitope specific phosphopeptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 106 kDa.
1/50 - 1/100.
Function May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Tissue specificity Ubiquitous.
Sequence similarities Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.
Cellular localization Membrane.
Information by UniProt
Choline phosphatase 2 antibody
EC 220.127.116.11 antibody
hPLD 2 antibody
Anti-Phospholipase D2 (phospho Y169) antibody images
Western blot - Phospholipase D2 (phospho Y169) antibody (ab78907)
All lanes : Anti-Phospholipase D2 (phospho Y169) antibody (ab78907) at 1/500 dilution Lane 1 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) Lane 2 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size : 106 kDa Observed band size : 94 kDa ( why is the actual band size different from the predicted?) Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Phospholipase D2 (phospho Y169) antibody (ab78907)
Immunohistochemical analysis of Phospholipase D2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.
References for Anti-Phospholipase D2 (phospho Y169) antibody (ab78907)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"