Overview

  • Product nameAnti-Phosphorylase B antibody
    See all Phosphorylase B primary antibodies
  • Description
    Rabbit polyclonal to Phosphorylase B
  • SpecificityDetects endogenous levels of Phosphorylase B protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from internal of human Phosphorylase B

  • Positive control
    • Extracts from K562 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab69586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
ELISA 1/40000.

Target

  • FunctionPhosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
  • PathwayGlycan biosynthesis; glycogen metabolism.
  • Involvement in diseaseGlycogen storage disease 9B
  • Sequence similaritiesBelongs to the phosphorylase b kinase regulatory chain family.
  • Post-translational
    modifications
    Ser-701 is probably phosphorylated by PKA.
    Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781E15103 antibody
    • FLJ41698 antibody
    • KPBB_HUMAN antibody
    • PHKB antibody
    • Phosphorylase b kinase regulatory subunit beta antibody
    • Phosphorylase kinase B antibody
    • Phosphorylase kinase beta polypeptide antibody
    • Phosphorylase kinase beta subunit antibody
    • Phosphorylase kinase subunit beta antibody
    see all

Anti-Phosphorylase B antibody images

  • All lanes : Anti-Phosphorylase B antibody (ab69586) at 1/500 dilution

    Lane 1 : extracts from K562 cells, without immunising peptide
    Lane 2 : extracts from K562 cells, with immunising peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 121 kDa
    Observed band size : 121 kDa

References for Anti-Phosphorylase B antibody (ab69586)

ab69586 has not yet been referenced specifically in any publications.

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