The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
FunctionPhosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
PathwayGlycan biosynthesis; glycogen metabolism.
Involvement in diseaseGlycogen storage disease 9B
Sequence similaritiesBelongs to the phosphorylase b kinase regulatory chain family.
Post-translational modificationsSer-701 is probably phosphorylated by PKA. Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.