Recombinant
RabMAb

Anti-Phosphorylase B antibody [EPR9035(2)] (ab181360)

Overview

  • Product name
    Anti-Phosphorylase B antibody [EPR9035(2)]
    See all Phosphorylase B primary antibodies
  • Description
    Rabbit monoclonal [EPR9035(2)] to Phosphorylase B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Phosphorylase B aa 650-750. The exact sequence is proprietary.
    Database link: Q93100

  • Positive control
    • Human fetal muscle, K562, HepG2, and Human fetal heart lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 121 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
    • Pathway
      Glycan biosynthesis; glycogen metabolism.
    • Involvement in disease
      Glycogen storage disease 9B
    • Sequence similarities
      Belongs to the phosphorylase b kinase regulatory chain family.
    • Post-translational
      modifications
      Ser-701 is probably phosphorylated by PKA.
      Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781E15103 antibody
      • FLJ41698 antibody
      • KPBB_HUMAN antibody
      • PHKB antibody
      • Phosphorylase b kinase regulatory subunit beta antibody
      • Phosphorylase kinase B antibody
      • Phosphorylase kinase beta polypeptide antibody
      • Phosphorylase kinase beta subunit antibody
      • Phosphorylase kinase subunit beta antibody
      see all

    Images

    • All lanes : Anti-Phosphorylase B antibody [EPR9035(2)] (ab181360) at 1/1000 dilution

      Lane 1 : Human fetal muscle lysates
      Lane 2 : K562 cell lysates
      Lane 3 : HepG2 cell lysates
      Lane 4 : Human fetal heart lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 121 kDa

    References

    ab181360 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab181360.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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