Anti-Phosphoserine antibody [3C171] (ab17465)

Overview

  • Product nameAnti-Phosphoserine antibody [3C171]
    See all Phosphoserine primary antibodies
  • Description
    Mouse monoclonal [3C171] to Phosphoserine
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Phosphoserine conjugated to Keyhole Limpet Haemocyanin (KLH).

Properties

Applications

Our Abpromise guarantee covers the use of ab17465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000 - 1/16000.
WB 1/500 - 1/1000.

(colorimetric). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.

ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody [3C171] images

  • ICC/IF image of ab17465 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17465, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot of canine brain hippocampus homogenates (10 ug loaded per lane for 3 different samples) separated on SDS-PAGE and blotted with ab17465.

References for Anti-Phosphoserine antibody [3C171] (ab17465)

This product has been referenced in:
  • Bhatt S  et al. Phosphorylation by p38 mitogen-activated protein kinase promotes estrogen receptor a turnover and functional activity via the SCF(Skp2) proteasomal complex. Mol Cell Biol 32:1928-43 (2012). Read more (PubMed: 22431515) »
  • Almeida S  et al. Dysregulation of CREB activation and histone acetylation in 3-nitropropionic acid-treated cortical neurons: prevention by BDNF and NGF. Neurotox Res 17:399-405 (2010). WB ; Rat . Read more (PubMed: 19779956) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (Macrophage)
Loading amount 10 µg
Specification Macrophage
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Dec 22 2006

Thank you for your enquiry. I was able to obtain an image. I'll send it to you in a separate email as I cannot attach it here. You will see that the antibody was tested at 1:200 in a Drosophila melanogaster whole tissue lysate (40ug/lane). There ...

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Thank you for your enquiry. I have updated the specificity statement for ab17465 with the following information from the source of the antibody: Recognizes phosphorylated serine both as free amino acid and when coupled to carriers such as BSA or ...

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I'm sorry to hear you are having problems with ab17465. Could you please clarify what you mean with "no signal despite positive controls on western"? Did the antibody work on cell lysate in WB and stop working when your protein of interest was immu...

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The immunogen was phosphoserine-KLH and the antibody recognizes free phosphoserine or phosphoserine conjugated to a carrier such as KLH or BSA. There is no immunizing peptide; just a phosphorylated amino acid.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"