The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml.
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Anti-Phosphoserine antibody images
Western blot - Anti-Phosphoserine antibody (ab125277)
All lanes : Anti-Phosphoserine antibody (ab125277) at 1/500 dilution
Lane 1 : A431 cell lysate (EGF stimulated) Lane 2 : A431 cell lysate (EGF stimulated) preincubated with Phosphoserine Lane 3 : A431 cell lysate (EGF stimulated) preincubated with Phosphotyrosine
References for Anti-Phosphoserine antibody (ab125277)
has not yet been referenced specifically in any publications.
Publishing research using ab125277? Please let us know so that we can cite the reference in this datasheet.
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