Overview

  • Product nameAnti-Phosphoserine antibody
    See all Phosphoserine primary antibodies
  • Description
    Rabbit polyclonal to Phosphoserine
  • SpecificityRecognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data.
  • Tested applicationsSuitable for: ELISA, IP, WB, IHC - Wholemount, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    BSA and KLH-phosphoserine conjugates.

  • Positive control
    • Mouse brain extract for Western Blotting. Synthetic phosphopeptide or phosvitin for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
IP Use at 10-0.25 µg/mg of lysate. Use at 10µg/0.25 mg of chromatography fractionated protein sample.
WB Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
IHC - Wholemount 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody images

  • All lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)

    Lane 1 : Whole cell lysate of monkey COS7 cells
    Lane 2 : Whole cell lysate of monkey COS7 cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution

    This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • ab9332 staining Phosphoserine in Human osteosarcoma SAOS-2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized in 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 1% BSA) for 1 hour. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-Phosphoserine antibody (ab9332)

This product has been referenced in:
  • Davison G  et al. Zinc carnosine works with bovine colostrum in truncating heavy exercise-induced increase in gut permeability in healthy volunteers. Am J Clin Nutr 104:526-36 (2016). Read more (PubMed: 27357095) »
  • Nakamura S  et al. Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation. Sci Rep 5:13474 (2015). Read more (PubMed: 26310847) »

See all 28 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (HEPATOCYTES)
Specification HEPATOCYTES
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2014

ab9332 is an anti-phosphoserine antibody and its immunogen is a KLH-phosphoserine conjugated to BSA. Since this antibody isn't specific for a particular protein but rather phosphoserine (a single modified amino acid), the species in which this antibody...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rabbit Recombinant protein (CDK8/CycC)
Specification CDK8/CycC
Type Indirect
Username

Abcam user community

Verified customer

Submitted Dec 13 2012

Thank you for contacting us.

Please find attached the requested data.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abr...

Read More

Thank you for your enquiry.

I can confirm that these two antibodies (ab9319 and ab9332) recognize modified amino acid residues (phosphotyrosine and phosphoserine respectively) on any proteins including murine samples. You are correct that mou...

Read More

Thank you for your enquiry. I am pleased to hear the customerwould like to make use of our testing discount offer and test ab9332 in IHC-Fr.

DISCOUNT CODE: #####
Expiration date: ######

This code will give you: 1 free primary...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Osteosarcoma SAOS-2)
Specification Osteosarcoma SAOS-2
Fixative Paraformaldehyde
Permeabilization Yes - PBS triton .25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 03 2012

Thank you for contacting us.

I am very glad to be of service in helping you to find the correct product for your needs. Ab9332 is guaranteed by our Abpromise to work in WB on mouse tissues.We will be pleased to provide scientific support, ...

Read More

We do offer two products which may meet your needs, ab17464 and ab117253.

While not truly Pan phospho Ser/ThrAb14764 detects phosphoserine or phosphothreonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phen...

Read More

1-10 of 33 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"