Overview

  • Product name
    Anti-Phosphoserine antibody
    See all Phosphoserine primary antibodies
  • Description
    Rabbit polyclonal to Phosphoserine
  • Specificity
    Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data.
  • Tested applications
    Suitable for: ELISA, IP, WB, IHC - Wholemount, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    BSA and KLH-phosphoserine conjugates.

  • Positive control
    • Mouse brain extract for Western Blotting. Synthetic phosphopeptide or phosvitin for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
IP Use at 10-0.25 µg/mg of lysate. Use at 10µg/0.25 mg of chromatography fractionated protein sample.
WB Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
IHC - Wholemount 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Images

  • All lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)

    Lane 1 : Whole cell lysate of monkey COS7 cells
    Lane 2 : Whole cell lysate of monkey COS7 cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution

    This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • ab9332 staining Phosphoserine in Human osteosarcoma SAOS-2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized in 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 1% BSA) for 1 hour. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies

    The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.

    The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.

    Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.

References

This product has been referenced in:
  • Xu X  et al. DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6. Nat Commun 8:15164 (2017). WB ; Human . Read more (PubMed: 28447620) »
  • Tan H  et al. HILI destabilizes microtubules by suppressing phosphorylation and Gigaxonin-mediated degradation of TBCB. Sci Rep 7:46376 (2017). Read more (PubMed: 28393858) »

See all 50 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Estrogen Receptor peptides)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
2 µg
Specification
Estrogen Receptor peptides
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Emiliya Storman

Verified customer

Submitted Dec 07 2017

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (HEPATOCYTES)
Specification
HEPATOCYTES
Treatment
20 ug/ml poly(I:C)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2014

ab9332 is an anti-phosphoserine antibody and its immunogen is a KLH-phosphoserine conjugated to BSA. Since this antibody isn't specific for a particular protein but rather phosphoserine (a single modified amino acid), the species in which this antibody...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rabbit Recombinant protein (CDK8/CycC)
Specification
CDK8/CycC
Type
Indirect
Username

Abcam user community

Verified customer

Submitted Dec 13 2012

Thank you for contacting us.

Please find attached the requested data.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abr...

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Thank you for your enquiry.

I can confirm that these two antibodies (ab9319 and ab9332) recognize modified amino acid residues (phosphotyrosine and phosphoserine respectively) on any proteins including murine samples. You are correct that mou...

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Thank you for your enquiry. I am pleased to hear the customerwould like to make use of our testing discount offer and test ab9332 in IHC-Fr.

DISCOUNT CODE: #####
Expiration date: ######

This code will give you: 1 free primary...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Osteosarcoma SAOS-2)
Specification
Osteosarcoma SAOS-2
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS triton .25%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 03 2012

Thank you for contacting us.

I am very glad to be of service in helping you to find the correct product for your needs. Ab9332 is guaranteed by our Abpromise to work in WB on mouse tissues.We will be pleased to provide scientific support, ...

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