BSA and KLH-phosphoserine conjugates.
Our Abpromise guarantee covers the use of ab9332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 µg/ml.|
|IP||Use at 10-0.25 µg/mg of lysate. Use at 10µg/0.25 mg of chromatography fractionated protein sample.|
|WB||Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.|
|IHC - Wholemount||1/200.|
|ICC/IF||Use at an assay dependent concentration.|
This image is courtesy of an Abreview submitted by Karin Birkenkamp-DemtroederBlocking Step: 10% Milk for 1 hour at room temperature