Overview

  • Product name
    Anti-Phosphoserine antibody (Biotin)
    See all Phosphoserine primary antibodies
  • Description
    Rabbit polyclonal to Phosphoserine (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    BSA and KLH-phosphoserine conjugates.

  • Positive control
    • Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.
IP Use at an assay dependent dilution.
ELISA Use a concentration of 0.5 µg/ml.

Target

  • Relevance
    Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

References

This product has been referenced in:
  • Zhang C  et al. Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner. Sci Rep 7:3769 (2017). WB ; Human . Read more (PubMed: 28630428) »
  • Phillips JE  et al. Glucocorticoid-induced osteogenesis is negatively regulated by Runx2/Cbfa1 serine phosphorylation. J Cell Sci 119:581-91 (2006). WB . Read more (PubMed: 16443755) »

See all 2 Publications for this product

Customer reviews and Q&As

All the information that we have regarding ab9335 is located on our online datasheet. Ab9335 specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins, and for a positive control it is recommended to use mouse brain ex...

Read More

Thank you for your enquiry. The anti-phosphoserine antibodies will not cross-react with phosphotidylserine and other phospholipids.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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