Anti-Phosphoserine antibody [PSR-45] (ab6639)

Overview

  • Product nameAnti-Phosphoserine antibody [PSR-45]
    See all Phosphoserine primary antibodies
  • Description
    Mouse monoclonal [PSR-45] to Phosphoserine
  • SpecificityMonoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.
  • Tested applicationsSuitable for: Dot blot, IP, Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    phospho-serine

    conjugated to KLH

  • Positive control
    • WB: rat brain cortex lysate ( a main 50kDa band is seen by detection with biotin-avidin AEC staining) ELISA: phosphoserine conjugated to BSA
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lot is from ascites and is still in stock as of 18th October 2016  - GR197414. Lot numbers higher than GR197414 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab6639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
IP 1/2000.
Indirect ELISA 1/4000.
WB 1/500 - 1/1000. Can be blocked with Synthetic Phosphoserine protein (BSA) (ab100812). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Stronger blocking agent percentage is recommended (e.g. 10%), in addition to varying loading amounts and shorter incubation time (1-2h RT)

Target

  • RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody [PSR-45] images

  • Anti-Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg

    Secondary
    Sheep anti mouse (HRP) at 1/2000 dilution

    Performed under reducing conditions.

    See Abreview

  • ab6639 used in Immunoprecipitation.
    Whole cell lysate prepared from murine mammary cells was loaded at 250µg.
    Cells were treated with 5ng/ml TGF beta for varying time periods.
    Protein A was used for the Immunoprecipitation step.
    ab6639 used at a 1/2000 dilution for 10 hours at 4°C.
    Western blot was performed with an HRP anti-PCBP1 polyclonal antibody, 1/10000 dilution.

    See Abreview

References for Anti-Phosphoserine antibody [PSR-45] (ab6639)

This product has been referenced in:
  • Kusumoto-Matsuo R  et al. Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks. Aging (Albany NY) 6:70-81 (2014). WB . Read more (PubMed: 24429382) »
  • Cho KI  et al. Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis. J Biol Chem 289:4600-25 (2014). WB . Read more (PubMed: 24403063) »

See all 13 Publications for this product

Product Wall

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Yes, the use of PBS is a good point. In case of using anti-phospho antibodies this can actually cause high background due to the presence of phosphate in the buffer. I would sugg...

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I am sorry to hear that the 2 antibodies have not been working as expected.

Your protocol looks fine in general. However, both antibodies are directed against ANY phospho-site on a serine or threonine o...

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I am sorry to confirm that ab6639 Phosphoserine antibody [PSR-45] has only been tested in rat brain tissue bythe originator, and so we have no images for human samples.

There have, h...

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To our knowledge this product has not been tested in IHC. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
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Based on the information that you have provided, I do not think that there is any protocol tips that would help resolve the issue, as I think you have tried everything.

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab76016. To check the status of the order please contact our Customer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"