Anti-Phosphoserine/threonine antibody (ab17464)


  • Product nameAnti-Phosphoserine/threonine antibody
    See all Phosphoserine/threonine primary antibodies
  • Description
    Rabbit polyclonal to Phosphoserine/threonine
  • Tested applicationsSuitable for: ICC/IF, ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide.

  • Positive control
    • Calyculin A treated A431 cells



Our Abpromise guarantee covers the use of ab17464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
ELISA 1/1000.
IP 1/100.
WB 1/1000.

Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.


  • RelevanceA hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The Phospho-(Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.
  • Alternative names
    • Phosphoserine antibody
    • Phosphothreonine antibody

Anti-Phosphoserine/threonine antibody images

  • All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/500 dilution

    Lane 1 : Whole tissue lysate prepared from murine heart (control)
    Lane 2 : Whole tissue lysate prepared from murine heart (control)
    Lane 3 : Whole tissue lysate prepared from murine heart (PKC)
    Lane 4 : Whole tissue lysate prepared from murine heart (PKC)

    Lysates/proteins at 50 µg per lane.

    HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
    developed using the ECL technique

    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Exposure time : 5 minutes

    Image courtesy of an anonymous Abreview.

    The lower blot is to detect total mybpc3. It was probed with a non-abcam anti-mybpc3 antibody.

    See Abreview

  • ab17464 staining Phosphoserine/threonine in murine cardiomyocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 1% Triton X-100, blocked with 10% horse serum for 1 hour at room temperature and then incubated with ab17464 at a 1/100 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

  • Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.

    To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
  • All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/1000 dilution

    Lane 1 : Human THP-1 Whole Cell Extract
    Lane 2 : Mouse Total Lung Extract

    Lysates/proteins at 10 µg per lane.

    Alkaline phosphatase conjugated goat anti-rabbit antibody
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-Phosphoserine/threonine antibody (ab17464) at 1/1000 dilution

    Lane 1 : calyculin A treated A431 cells
    Lane 2 : calyculin A treated A431 cells

References for Anti-Phosphoserine/threonine antibody (ab17464)

This product has been referenced in:
  • Wang Y  et al. Type II cyclic guanosine monophosphate-dependent protein kinase inhibits Rac1 activation in gastric cancer cells. Oncol Lett 10:502-508 (2015). Human . Read more (PubMed: 26171059) »
  • Kotla S & Rao GN Reactive Oxygen Species (ROS) Mediate p300-dependent STAT1 Protein Interaction with Peroxisome Proliferator-activated Receptor (PPAR)-? in CD36 Protein Expression and Foam Cell Formation. J Biol Chem 290:30306-20 (2015). Read more (PubMed: 26504087) »

See all 10 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 26 2014

The concentration of ab17464 Anti-Phosphoserine/threonine antibody lot GR112407 is 0.075mg

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Treatment UV irradiation
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 13 2013

Thank you for contacting us.

The ab9337 recognizes proteins phosphorylated on threonine residues. It does not cross-react with phosphotyrosine.

For ab15556, this antibody recognizes serine, threonine, and tyrosine phosphorylated pr...

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