Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers.
Our Abpromise guarantee covers the use of ab15556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||1/200.|
|Functional Studies||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Milk derived blocking solutions reportedly contain phosphoproteins that may inhibit phosphoamino acid antibody binding and therefore should be avoided.|
|IP||Use at 2 µg/mg of lysate.|
|IHC-P||1/20. Boil tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20421451|