• Product nameAnti-Phosphothreonine antibody
    See all Phosphothreonine primary antibodies
  • Description
    Rabbit polyclonal to Phosphothreonine
  • SpecificityRecognize proteins phosphorylated on threonine residues. Not cross-reacted with phosphotyrosine.
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH.

  • Positive control
    • Use mouse spleen lysate treated with sodium vanadate or mouse brain lysate for WB. Synthetic phosphopeptide (phosphorylated on threonine) for ELISA.



Our Abpromise guarantee covers the use of ab9337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml.
IP Use a concentration of 10 µg/ml. Use at a concentration of 10 µg/mg. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.
ELISA Use a concentration of 0.5 µg/ml.
IHC-Fr Use at an assay dependent concentration.


  • RelevancePhosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.

Anti-Phosphothreonine antibody images

  • Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
  • Antibody Capture ELISA
    Label: immobilized antigen

References for Anti-Phosphothreonine antibody (ab9337)

This product has been referenced in:
  • Davison G  et al. Zinc carnosine works with bovine colostrum in truncating heavy exercise-induced increase in gut permeability in healthy volunteers. Am J Clin Nutr 104:526-36 (2016). Read more (PubMed: 27357095) »
  • Nakamura S  et al. Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation. Sci Rep 5:13474 (2015). Read more (PubMed: 26310847) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Human Cell lysate - whole cell (Immunoprecipitad protein phosphorylated at Threoni)
Specification Immunoprecipitad protein phosphorylated at Threoni
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Eleni Petsalaki

Verified customer

Submitted Feb 27 2014

1) The antibody has been tested for phosphoserine in a competitive ELISA. The cross-reactivity is about 10% to phosphoserine, 25% to phosphotyrosine.

2) The relative affinity was not tested.

3) 125 ng of ...

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Thank you for contacting us.

The ab9337 recognizes proteins phosphorylated on threonine residues. It does not cross-react with phosphotyrosine.

For ab15556, this antibody recognizes serine, threonine, and tyrosine phosphorylated pr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Purified protein (Hepa1-6 hepatoma cells)
Loading amount 1000 µg
Specification Hepa1-6 hepatoma cells
Treatment 1 nM insulin for 15 min/no insulin
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A431)
Loading amount 1e+006 cells
Specification A431
Treatment calyculin A 1 h
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Dec 15 2006

You may want to try a nitrocellulose membrane instead - nitrocellulose membranes are considered to give less background compared to PVDF membranes. Please let me know if you have any additional questions.

Thank you for your patience. I have enquired with the originator of this antibody to see if there have been any other complaints regarding the lot that you received and I'm still waiting to hear back. Did you try decreasing the amount of primary that y...

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Thank you very much for the details that you have provided and for your patience. I think that the problem is due to the blocking step. For phospho-specific antibodies you should use BSA as the blocking agent, and never use milk. This is because kinase...

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We did find the anti-phoserine and anti-phosphothreonine antibodies bind to phosvitin, a known phosphoprotein. The anti-phosphoserine/threonine also binds to phosho-serine/threonine in a peptide. I am very sorry to inform you that we do not stoc...

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