Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)

Overview

  • Product name
    Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody
    See all Phosphothreonine-Proline / Phosphoserine-Proline primary antibodies
  • Description
    Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline
  • Specificity
    This antibody specifically reacts to proteins containing phosphothreonine-proline motifs, it also reacts to the phosphoserine-proline motif to a similar degree (pT-P and pS-P motif). The antibody does not react to phosphothreonine, phosphoserine or phosphotyrosine. The antibody will selectively recognize the phosphothreonine-proline motif of MBP, which is the site of phosphorylation by ERK1/ERK2.
  • Tested applications
    Suitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule: TpP conjugated to KLH.

  • Positive control
    • WB: Mouse brain lysate ELISA: Phosvitin or casein
  • General notes


    The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Immunoaffinity chromatography with phosphothreonine-proline on agarose, then immuno-absorption with threonine-proline-NH2 on agarose.
  • Primary antibody notes
    The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23269672

References

This product has been referenced in:
  • Godínez-Palma SK  et al. Two maize Kip-related proteins differentially interact with, inhibit and are phosphorylated by cyclin D-cyclin-dependent kinase complexes. J Exp Bot 68:1585-1597 (2017). Read more (PubMed: 28369656) »
  • Mukherjee S  et al. Phosphorylation of Ku70 subunit by cell cycle kinases modulates the replication related function of Ku heterodimer. Nucleic Acids Res 44:7755-65 (2016). IP . Read more (PubMed: 27402161) »

See all 6 Publications for this product

Customer reviews and Q&As

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I am very pleased to hear you would like to accept our offer and test ab9344 in IF. The code above will give you £227 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IF and includ...

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Thank you for your reply.

Kate is currently not in the office but I will try to help with your enquiry as best I can. If you were to test this antibody in IF you would be eligible to take part in the Abtrial testing program.

The way t...

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Thank you for your message which has been forwarded to the scientific support team.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications...

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Thank you for contacting Abcam. The antibody ab9344 is guaranteed to work in ELISAs and so would most probably work in the application that you intend to use it for, however you would have to work out the exact condition for you experiment, but...

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Thank you for contacting us. We have anti glycine or anti- proline antibodies in our catalogue. The catalogue numbers are ab9344, ab9345, ab37067, ab9442 and ab37087. We unfortunately do not have antibodies available specifically raised against ...

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It is not tested. If there is no steric hindrance, it should be able to do so.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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