Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)


  • Product nameAnti-Phosphothreonine-Proline / Phosphoserine-Proline antibody
    See all Phosphothreonine-Proline / Phosphoserine-Proline primary antibodies
  • Description
    Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline
  • SpecificityThis antibody specifically reacts to proteins containing phosphothreonine-proline motifs, it also reacts to the phosphoserine-proline motif to a similar degree (pT-P and pS-P motif). The antibody does not react to phosphothreonine, phosphoserine or phosphotyrosine. The antibody will selectively recognize the phosphothreonine-proline motif of MBP, which is the site of phosphorylation by ERK1/ERK2.
  • Tested applicationsSuitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule: TpP conjugated to KLH.

  • Positive control
    • WB: Mouse brain lysate ELISA: Phosvitin or casein
  • General notes

    The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesImmunoaffinity chromatography with phosphothreonine-proline on agarose, then immuno-absorption with threonine-proline-NH2 on agarose.
  • Primary antibody notesThe antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab9344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23269672

References for Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)

This product has been referenced in:
  • Jonkers W  et al. HAM-5 functions as a MAP kinase scaffold during cell fusion in Neurospora crassa. PLoS Genet 10:e1004783 (2014). WB . Read more (PubMed: 25412208) »
  • Yu J  et al. Modulation of fatty acid synthase degradation by concerted action of p38 MAP kinase, E3 ligase COP1, and SH2-tyrosine phosphatase Shp2. J Biol Chem 288:3823-30 (2013). IP ; Mouse . Read more (PubMed: 23269672) »

See all 4 Publications for this product

Product Wall


I am very pleased to hear you would like to accept our offer and test ab9344 in IF. The code above will give you £227 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IF and includ...

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Thank you for your reply.

Kate is currently not in the office but I will try to help with your enquiry as best I can. If you were to test this antibody in IF you would be eligible to take part in the Abtrial testing program.

The way t...

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Thank you for your message which has been forwarded to the scientific support team.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications...

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Thank you for contacting Abcam. The antibody ab9344 is guaranteed to work in ELISAs and so would most probably work in the application that you intend to use it for, however you would have to work out the exact condition for you experiment, but...

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Thank you for contacting us. We have anti glycine or anti- proline antibodies in our catalogue. The catalogue numbers are ab9344, ab9345, ab37067, ab9442 and ab37087. We unfortunately do not have antibodies available specifically raised against ...

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It is not tested. If there is no steric hindrance, it should be able to do so.