Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (HRP) (ab9345)

Overview

  • Product name
    Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (HRP)
    See all Phosphothreonine-Proline / Phosphoserine-Proline primary antibodies
  • Description
    Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule: TpP conjugated to KLH.

  • Positive control
    • Mouse brain extract for immunoblotting. Phosvitin or casein for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Images

  • Auto-phosphorylation of the crude mouse brain extracts ( 50 ug total crude protein) as detected with anti-pT-P antibodies. (Alkaline phosphatase anti-rabbit secondary system.)
    Lane A: treated with ATP (0.25 mM) 5 min
    Lane B: Without ATP treatment (lower band : small amount of phosphorylated protein)

References

This product has been referenced in:
  • Kendall RT  et al. The beta-arrestin pathway-selective type 1A angiotensin receptor (AT1A) agonist [Sar1,Ile4,Ile8]angiotensin II regulates a robust G protein-independent signaling network. J Biol Chem 286:19880-91 (2011). WB . Read more (PubMed: 21502318) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. We have anti glycine or anti- proline antibodies in our catalogue. The catalogue numbers are ab9344, ab9345, ab37067, ab9442 and ab37087. We unfortunately do not have antibodies available specifically raised against ...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab9345 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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