Western blot - Phosphothreonine-Proline / Phosphoserine-Proline antibody (HRP) (ab9345)
Auto-phosphorylation of the crude mouse brain extracts ( 50 ug total crude protein) as detected with anti-pT-P antibodies. (Alkaline phosphatase anti-rabbit secondary system.)
Lane A: treated with ATP (0.25 mM) 5 min
Lane B: Without ATP treatment (lower band : small amount of phosphorylated protein)
References for Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (HRP) (ab9345)
This product has been referenced in:
Kendall RT et al. The beta-arrestin pathway-selective type 1A angiotensin receptor (AT1A) agonist [Sar1,Ile4,Ile8]angiotensin II regulates a robust G protein-independent signaling network. J Biol Chem286:19880-91 (2011).
Read more (PubMed: 21502318) »