Anti-Phosphothreonine-X-Phosphotyrosine antibody (ab9341)

Overview

  • Product nameAnti-Phosphothreonine-X-Phosphotyrosine antibody
    See all Phosphothreonine-X-Phosphotyrosine primary antibodies
  • Description
    Rabbit polyclonal to Phosphothreonine-X-Phosphotyrosine
  • SpecificityELISA test indicated that this antibody specifically reacted to peptide containing pT-G-pY, pT-E-pY sequence, but weakly reacted to pT-P-pY sequence. The antibody does not react with phosphothreonine and phosphoserine but reacts weakly with phosphotyrosine.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    KLH-phosphothreonine-lysine-phosphotyrosine (N-acetyl-pT-K-pY-NH2) peptide conjugate.

  • Positive control
    • Mouse brain extract for immunoblotting. Synthetic pT-X-pY containing phosphopeptide for ELISA.
  • General notes

    Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesImmunoaffinity chromatography with N-acetyl-phosphothreonine-lysine-phosphotyrosine amide agarose, absorbed with N-acetyl-threonine-lysine-tyrosine agarose.
  • Primary antibody notesAntigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250.
IP Use at an assay dependent dilution.
ELISA 1/500.

Anti-Phosphothreonine-X-Phosphotyrosine antibody images

  • Immunoblotting of a human fibroblast cell lysate (4 hour UV treated and non-treated) with anti-pT-X-pY antibodies.
  • Immunoblotting of protein extracts with anti-pT-X-pY antibodies

    A: Extract from Mouse Brain
    B: Extract from Mouse Brain teated with Okadaic Acid(0.1 uM, 30 min).
    C: Lysate of Melanoma Cell
    D: Lysate of Melanoma Cell treated with Okadaic Acid.

References for Anti-Phosphothreonine-X-Phosphotyrosine antibody (ab9341)

This product has been referenced in:
  • Ma Z & Yu G Phosphorylation of mitogen-activated protein kinase (MAPK) is required for cytokinesis and progression of cell cycle in tobacco BY-2 cells. J Plant Physiol 167:216-21 (2010). Read more (PubMed: 19765858) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"