Anti-Phosphothreonine-X-Phosphotyrosine antibody (HRP) (ab9342)

Overview

  • Product name
    Anti-Phosphothreonine-X-Phosphotyrosine antibody (HRP)
    See all Phosphothreonine-X-Phosphotyrosine primary antibodies
  • Description
    Rabbit polyclonal to Phosphothreonine-X-Phosphotyrosine (HRP)
  • Conjugation
    HRP
  • Specificity
    ELISA test indicated that this antibody specifically reacted to peptide containing pT-G-pY, pT-E-pY sequence, but weakly reacted to pT-P-pY sequence. The antibody does not react with phosphothreonine and phosphoserine but reacts weakly with phosphotyrosine.
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    KLH-phosphothreonine-lysine-phosphotyrosine (N-acetyl-pT-K-pY-NH2) peptide conjugate.

  • Positive control
    • Mouse brain extract for immunoblotting. Synthetic pT-X-pY containing phosphopeptide for ELISA.
  • General notes

    Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Phosphate-buffered Saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Immunoaffinity chromatography with N-acetyl-phosphothreonine-lysine-phosphotyrosine amide agarose, absorbed with N-acetyl-threonine-lysine-tyrosine agarose.
  • Primary antibody notes
    Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250.
IP Use at an assay dependent concentration.
ELISA 1/500.

Images

  • Immunoblotting of a human fibroblast cell lysate (4 hour UV treated and non-treated) with anti-pT-X-pY antibodies.
  • Immunoblotting of protein extracts with anti-pT-X-pY antibodies

    A: Extract from Mouse Brain
    B: Extract from Mouse Brain teated with Okadaic Acid(0.1 uM, 30 min).
    C: Lysate of Melanoma Cell
    D: Lysate of Melanoma Cell treated with Okadaic Acid.

References

This product has been referenced in:
  • Loomis Z  et al. Hemoglobin induced cell trauma indirectly influences endothelial TLR9 activity resulting in pulmonary vascular smooth muscle cell activation. PLoS One 12:e0171219 (2017). Read more (PubMed: 28152051) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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