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Rabbit polyclonal to Phosphothreonine-X-Phosphotyrosine (HRP)
ELISA test indicated that this antibody specifically reacted to peptide containing pT-G-pY, pT-E-pY sequence, but weakly reacted to pT-P-pY sequence. The antibody does not react with phosphothreonine and phosphoserine but reacts weakly with phosphotyrosine.
KLH-phosphothreonine-lysine-phosphotyrosine (N-acetyl-pT-K-pY-NH2) peptide conjugate.
Mouse brain extract for immunoblotting. Synthetic pT-X-pY containing phosphopeptide for ELISA.
Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's.
Shipped at 4°C. Store at +4°C.
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Immunogen affinity purified
Immunoaffinity chromatography with N-acetyl-phosphothreonine-lysine-phosphotyrosine amide agarose, absorbed with N-acetyl-threonine-lysine-tyrosine agarose.
Primary antibody notes
Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"