Anti-Phosphotyrosine antibody [2Q267] (ab17285)

Overview

  • Product nameAnti-Phosphotyrosine antibody [2Q267]
    See all Phosphotyrosine primary antibodies
  • Description
    Mouse monoclonal [2Q267] to Phosphotyrosine
  • SpecificityBinds specifically and with a high affinity to proteins with phosphorylated tyrosine residues. Binding can be blocked by phosphotyrosine and phenyl phosphate (IC50:10e-6M, ELISA). Does not crossreact with tyrosine, phosphoserine, phosphothreonine, sulphotyrosine (sulfated (23-25) cholecystokinine) or Na-benzoate
  • Tested applicationsSuitable for: ELISA, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule (Hapten O-phopshotyramine)

Properties

Applications

Our Abpromise guarantee covers the use of ab17285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.4 - 1 µg/ml.
IP Use a concentration of 100 µg/ml.
ICC Use a concentration of 5 - 10 µg/ml.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

References for Anti-Phosphotyrosine antibody [2Q267] (ab17285)

ab17285 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab17285.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"