Anti-Phosphotyrosine antibody (ab17302)

Overview

  • Product nameAnti-Phosphotyrosine antibody
    See all Phosphotyrosine primary antibodies
  • Description
    Rabbit polyclonal to Phosphotyrosine
  • SpecificityMinimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
  • Tested applicationsSuitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Affinity purified native tyrosine phosphorylated proteins (Human).

  • Positive control
    • A431 Cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab17302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/2000.
IP Use at an assay dependent dilution. Use 2.5 µg pwe IP reaction.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody images

  • All lanes : Anti-Phosphotyrosine antibody (ab17302)

    Lane 1 : Untreated parotid acinar cells
    Lane 2 : Parotid acinar cells with 50µM etoposide for 2 hours
    Lane 3 : Parotid acinar cells with 5mM H2O2 for 30 min

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

References for Anti-Phosphotyrosine antibody (ab17302)

ab17302 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Parotid acinar cells)
Loading amount 50 µg
Specification Parotid acinar cells
Treatment untreated (UT), + 50uM etoposide for 2 hours (E), + 5mM H2O2 for 30 min (H)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"