The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/10000.
1/1000 - 1/2000.
Use at an assay dependent dilution. Use 2.5 µg pwe IP reaction.
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Western blot - Phosphotyrosine antibody (ab17302)
All lanes : Anti-Phosphotyrosine antibody (ab17302)
Lane 1 : Untreated parotid acinar cells Lane 2 : Parotid acinar cells with 50µM etoposide for 2 hours Lane 3 : Parotid acinar cells with 5mM H2O2 for 30 min
Lysates/proteins at 50 µg per lane.
Secondary HRP-conjugated Donkey anti-Rabbit IgG at 1/10000 dilution