Overview

  • Product nameAnti-Phosphotyrosine antibody
    See all Phosphotyrosine primary antibodies
  • Description
    Rabbit polyclonal to Phosphotyrosine
  • Specificityab50722 recognizes tyrosine phosphorylated proteins. The antibody recognizes the pY-256 random peptide and phospho-L-tyrosine, but does not recognize phospho-L-serine, phospho-Lthreonine or L-tyrosine.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Phospho-L-tyrosine containing random peptide pY-256, conjugated to KLH.

  • Positive control
    • Total cell extract of A431 stimulated by human EGF
  • General notes


    Antibodies specific for phosphotyrosine are essential tools for the characterization of tyrosine phosphorylation in many signal transduction pathways. Polyclonal antibodies can offer advantages over monoclonals, especially in immunoprecipitation protocols, and can theoretically react with a broader range of phosphotyrosine-containing proteins.

Properties

Applications

Our Abpromise guarantee covers the use of ab50722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody images

  • All lanes : Anti-Phosphotyrosine antibody (ab50722) at 0.3 µg/ml

    Lane 1 : Whole cell extracts of untreated A431 cells
    Lane 2 : Whole cell extracts of EGF-treated A431 cells
    Lane 3 : Whole cell extracts of peroxyvanadate-treated A431 cells

    Secondary
    Goat Anti-Rabbit IgG-Peroxidase conjugate using a chemiluminescent substrate
    Developed using the ECL technique

    Predicted band size : 10 kDa
  • Plasma membrane and cytoplasmic staining A431 Cells were fixed with paraformaldheyde and stained with ab50722 at 1/100, followed by Goat anti-Rabbit IgG (H+L), FITC conjugate. Panel A: Serum-starved A431 cells stimulated with human EGF (1 µg/mL, 5 min, EGF human). Panel B: Untreated control A431 cells.

References for Anti-Phosphotyrosine antibody (ab50722)

ab50722 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"