Anti-Phosphotyrosine antibody (ab9319)
Key features and details
- Rabbit polyclonal to Phosphotyrosine
- Suitable for: ELISA, IP, WB, IHC-P, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies -
Description
Rabbit polyclonal to Phosphotyrosine -
Host species
Rabbit -
Specificity
Recognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine. -
Tested applications
Suitable for: ELISA, IP, WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin. Phospho-tyrosine KLH conjugates
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Positive control
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA. Human breast cancer tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.00
Preservative: 0.01% Sodium azide
Constituent: 0.114% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/1000.
|
|
IP |
Use at an assay dependent concentration. 10 µg/500 µg protein sample.
|
|
WB | (2) |
1/250.
|
IHC-P |
1/50.
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|
IHC-Fr |
1/50.
|
Notes |
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ELISA
1/1000. |
IP
Use at an assay dependent concentration. 10 µg/500 µg protein sample. |
WB
1/250. |
IHC-P
1/50. |
IHC-Fr
1/50. |
Target
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Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Images
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All lanes : Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/ml
Lane 1 : 5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum
Lane 2 : 15 ul of crude protein from HeLa cell lysate treated with fetal calf serum
Lane 3 : 5 ul of crude protein from HeLa cell lysate
Lane 4 : 15 ul of crude protein from HeLa cell lysate
Lysates/proteins at 2 mg/ml per lane. -
Immunohistochemistry analysis of human breast cancer with ab9319
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab9319 has been referenced in 10 publications.
- Arthur S et al. Unique Regulation of Coupled NaCl Absorption by Inducible Nitric Oxide in a Spontaneous SAMP1/YitFc Mouse Model of Chronic Intestinal Inflammation. Inflamm Bowel Dis 27:1804-1812 (2021). PubMed: 34019094
- Verheyen T et al. Spatial organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers. Nucleic Acids Res 48:5777-5787 (2020). PubMed: 32352518
- Ramani K et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation. Am J Pathol 188:640-655 (2018). PubMed: 29305319
- Duan ZZ et al. Protease activated receptor 1 (PAR1) enhances Src-mediated tyrosine phosphorylation of NMDA receptor in intracerebral hemorrhage (ICH). Sci Rep 6:29246 (2016). PubMed: 27385592
- Yosef E et al. Do Src Kinase and Caveolin Interact Directly with Na,K-ATPase? J Biol Chem 291:11736-50 (2016). PubMed: 27022017
- Nakamura S et al. Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation. Sci Rep 5:13474 (2015). PubMed: 26310847
- Shaw A et al. Spatial control of membrane receptor function using ligand nanocalipers. Nat Methods 11:841-6 (2014). PubMed: 24997862
- Debreuil J et al. Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum. J Biol Chem 287:19367-76 (2012). WB . PubMed: 22505718
- Zhong Z et al. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region. J Neurosci 30:15521-34 (2010). IP . PubMed: 21084607
- Moya A et al. Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization. J Biol Chem 283:25475-84 (2008). WB . PubMed: 18617510