Overview

  • Product nameAnti-Phosphotyrosine antibody
    See all Phosphotyrosine primary antibodies
  • Description
    Rabbit polyclonal to Phosphotyrosine
  • SpecificityRecognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine.
  • Tested applicationsSuitable for: ELISA, IP, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to Phosphotyrosine conjugated to Keyhole Limpet Haemocyanin (KLH). Iodoacetylphosphotyramine KLH conjugates

  • Positive control
    • Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA. Human breast cancer tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab9319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IP Use at an assay dependent concentration. 10 µg/500 µg protein sample.
WB 1/250.
IHC-P 1/50.
IHC-Fr 1/50.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody images

  • All lanes : Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/ml

    Lane 1 : 5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum
    Lane 2 : 15 ul of crude protein from HeLa cell lysate treated with fetal calf serum
    Lane 3 : 5 ul of crude protein from HeLa cell lysate
    Lane 4 : 15 ul of crude protein from HeLa cell lysate

    Lysates/proteins at 2 mg/ml per lane.

References for Anti-Phosphotyrosine antibody (ab9319)

This product has been referenced in:
  • Nakamura S  et al. Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation. Sci Rep 5:13474 (2015). Read more (PubMed: 26310847) »
  • Debreuil J  et al. Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum. J Biol Chem 287:19367-76 (2012). WB . Read more (PubMed: 22505718) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry.

I can confirm that these two antibodies (ab9319 and ab9332) recognize modified amino acid residues (phosphotyrosine and phosphoserine respectively) on any proteins including murine samples. You are correct that mou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell ((TF-1 (human, lymphoid, erythroleukemia) cells))
Specification (TF-1 (human, lymphoid, erythroleukemia) cells)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. Alison Taylor

Verified customer

Submitted Sep 25 2006

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested for application in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you ...

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1. Add cold acetone:lysate sample, v/v=9/1. eg: 100 uL smaple add 900 uL acetone. 2.Centrifuge at 5000 rpm for 5 min 3. Reconstitute with the precipitate with same volume of PBS then add 0.5% of SDS to the solution with 10% SDS stcok. 4. boil for 5 ...

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You could use the mouse fibroblast cells as positive control. First, remove the old medium and culture the cells in a serum-free medium for 24 hours. Then empty the dish again and treat the cell with the fetal calf serum media containing 1 mM Vanadi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"