Anti-Phosphotyrosine antibody (Biotin) (ab9331)

Overview

  • Product nameAnti-Phosphotyrosine antibody (Biotin)
    See all Phosphotyrosine primary antibodies
  • Description
    Rabbit polyclonal to Phosphotyrosine (Biotin)
  • ConjugationBiotin
  • SpecificityRecognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH.

  • Positive control
    • Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
  • Application notesELISA: 1/500.
    IP: Use at 16µg/mg of lysate. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
    WB: 1/125. Not recommended for WB of trace cellular phospho-protein. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    Anti-Phosphotyrosine antibody (Biotin) images

    • Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

    References for Anti-Phosphotyrosine antibody (Biotin) (ab9331)

    ab9331 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"