The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 16µg/mg of lysate. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
WB: 1/125. Not recommended for WB of trace cellular phospho-protein. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Anti-Phosphotyrosine antibody (Biotin) images
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)