Anti-Phosphotyrosine antibody (HRP) (ab9329)

Overview

  • Product nameAnti-Phosphotyrosine antibody (HRP)
    See all Phosphotyrosine primary antibodies
  • Description
    Rabbit polyclonal to Phosphotyrosine (HRP)
  • ConjugationHRP
  • SpecificityRecognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH.

  • Positive control
    • Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody (HRP) images

  • Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

References for Anti-Phosphotyrosine antibody (HRP) (ab9329)

This product has been referenced in:
  • McLaughlin NJ  et al. Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180:8192-203 (2008). WB . Read more (PubMed: 18523285) »

See 1 Publication for this product

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We do not have affinity data for this antibody. If you need an HRP-conjugated antibody, I recommend the mouse monoclonal ab16389:

Click here (or use the following: http://www.abcam.com/index.html?datas...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (CD4+ murine splenoyctes)
Loading amount 50000 cells
Specification CD4+ murine splenoyctes
Treatment anti-CD3 and anti-CD28-coated beads
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
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Submitted Nov 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"