Anti-Phosphotyrosine antibody [IG2] (ab5592)

Overview

  • Product nameAnti-Phosphotyrosine antibody [IG2]
    See all Phosphotyrosine primary antibodies
  • Description
    Mouse monoclonal [IG2] to Phosphotyrosine
  • Specificityab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab5592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/200.
WB 1/100 - 1/1000.
ICC 1/50 - 1/200.
Flow Cyt Use at an assay dependent concentration.
IP Use a concentration of 2 µg/ml.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody [IG2] images

  • Immunolocalization of phosphotyrosine in HeLa cells using ab5592.

References for Anti-Phosphotyrosine antibody [IG2] (ab5592)

ab5592 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"