Mouse monoclonal [PT-66] to Phosphotyrosine (Biotin)
SpecificityThis antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
References for Anti-Phosphotyrosine antibody [PT-66] (Biotin) (ab49667)
This product has been referenced in:
Dvir A et al. The inhibition of EGF-dependent proliferation of keratinocytes by tyrphostin tyrosine kinase blockers. J Cell Biol113:857-65 (1991).
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