Phosphotyrosine antibody [PY20] (ab10321)

Product Name 

Phosphotyrosine antibody [PY20]

Product type 

Primary antibodies

Description 

Mouse monoclonal [PY20] to Phosphotyrosine

Immunogen 

Phosphotyrosine conjugated to KLH.

Reacts with 
(species key)

Hu, Ms, Rat, AGMk

Specificity 

This antibody recognizes phospho-tyrosine and phosphotyrosine- containing proteins.

Tested applications 
(see key)

AP, ELISA, Flow Cyt, ICC/IF, IHC-FoFr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
AP: Use at a concentration of 4 mg/ml.
ELISA: Use at a concentration of 0.1 µg/ml.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: 1/200(see Abreview).
IHC-FoFr: Use at a concentration of 1 µg/ml.
IP: 1/400 (see review).
WB: Use at a concentration of 2 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Caution: The use of milk-containing buffers may interfere with the ability of phosphotyrosine antibodies to bind specific proteins of interest. Use BSA-containing buffers for blocking and incubating purposes and incubate for 1hr in blocking buffer.

Research areas 

Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Phosphatases

Relevance 

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

This is a standard clone used to detect phosphotyrosine.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

PY20

Isotype 

IgG2b

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Phosphotyrosine antibody [PY20] (ab10321)

  This product has been used in: (two most recent references)

Lemeer S et al. Endogenous phosphotyrosine signaling in zebrafish embryos. Mol Cell Proteomics 6:2088-99 (2007). PubMed: 17698882

Huang F et al. EGF receptor ubiquitination is not necessary for its internalization. Proc Natl Acad Sci U S A 104:16904-9 (2007). PubMed: 17940017

See all 20 publication references for this product.


If you publish research using ab10321 please let us know so that we can cite the reference on this datasheet.

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