Anti-Phosphotyrosine antibody [PY20] (Biotin) (ab16388)

Overview

  • Product nameAnti-Phosphotyrosine antibody [PY20] (Biotin)
    See all Phosphotyrosine primary antibodies
  • Description
    Mouse monoclonal [PY20] to Phosphotyrosine (Biotin)
  • ConjugationBiotin
  • SpecificityClone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
  • Tested applicationsSuitable for: WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Phosphotyrosine coupled to carrier protein

  • General notes

    This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

    The purified antibody is coupled through free primary amino groups to biotin. The binding site of the antibody was protected by the addition of phenyl phosphate prior to coupling.

Properties

Applications

Our Abpromise guarantee covers the use of ab16388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC
  • Application notes
    ELISA: 1/5000.
    ICC: 1/500.
    WB: 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    References for Anti-Phosphotyrosine antibody [PY20] (Biotin) (ab16388)

    ab16388 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Human Recombinant protein (YSPTSPS (CTD repeat) peptide)
    Specification YSPTSPS (CTD repeat) peptide
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
    Type Direct
    Username

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    Verified customer

    Submitted Mar 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"