Anti-Phosphotyrosine antibody [PY20] (HRP) (ab16389)

Overview

  • Product nameAnti-Phosphotyrosine antibody [PY20] (HRP)
    See all Phosphotyrosine primary antibodies
  • Description
    Mouse monoclonal [PY20] to Phosphotyrosine (HRP)
  • ConjugationHRP
  • Specificityab16389 specifically recognises phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Phosphotyrosine coupled to carrier protein.

  • Positive control
    • This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate.
  • General notes

    This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

    Alternative versions available:
    Anti-Phosphotyrosine antibody [PY20] (ab10321)
    Anti-Phosphotyrosine antibody (FITC) [PY20] (ab25416)

Properties

Applications

Our Abpromise guarantee covers the use of ab16389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody [PY20] (HRP) images

  • Anti-Phosphotyrosine antibody [PY20] (HRP) (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Anti-Phosphotyrosine antibody [PY20] (HRP) (ab16389) at 1/5000 dilution ((BLOCKED WITH 3% MILK)) + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

References for Anti-Phosphotyrosine antibody [PY20] (HRP) (ab16389)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application ELISA
Sample Mouse Cell (Neuroblastoma)
Specification Neuroblastoma
Type Sandwich (Detection)
Blocking step 3% BSA and 1% FBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Mar 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"