Anti-Phosphotyrosine antibody [PY20] (Rhodamine) (ab28346)

Overview

  • Product nameAnti-Phosphotyrosine antibody [PY20] (Rhodamine)
    See all Phosphotyrosine primary antibodies
  • Description
    Mouse monoclonal [PY20] to Phosphotyrosine (Rhodamine)
  • ConjugationRhodamine. Ex: 550nm, Em: 570nm
  • SpecificityAb28346 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
  • Tested applicationsSuitable for: ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Phosphotyrosine coupled to carrier protein.

  • General notes

    Ab28346 is especially useful for the detection of these phosphorylated tyrosine proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest. The antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

    The purified antibody was labeled by reaction with tetramethyl rhodamine (TAMRA) succinimidyl ester in the presence of phenyl phosphate (to protect the antibody binding site). The unreacted TAMRA succinimidyl ester was removed by molecular exclusion chromatography.

Properties

Applications

Our Abpromise guarantee covers the use of ab28346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 5 - 10 µg/ml.
WB 1/5000.

Target

  • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

References for Anti-Phosphotyrosine antibody [PY20] (Rhodamine) (ab28346)

ab28346 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"