Anti-Phosphotyrosine antibody [SP4] (ab47413)

Overview

  • Product nameAnti-Phosphotyrosine antibody [SP4]
    See all Phosphotyrosine primary antibodies
  • Description
    Mouse monoclonal [SP4] to Phosphotyrosine
  • SpecificityThis antibody recognizes tyrosine phosphorylated proteins.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    This monoclonal antibody is produced by immunizing mice with phospho-tyrosine conjugated to protein carriers.

  • Positive control
    • Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab47413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    Anti-Phosphotyrosine antibody [SP4] images

    • ab47413 (1/50) staining paraffin-embedded human breast carcinoma tissue (left). Right hand panel represents a negative control where ab47413 was pre-icubated with Phospho-tyrosine molecules.

    References for Anti-Phosphotyrosine antibody [SP4] (ab47413)

    ab47413 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"