Overview

  • Product name
  • Description
    Rabbit polyclonal to Phostensin
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 199-228 of Human Phostensin.

  • Positive control
    • Ramos cell lysate; Human spleen tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab103327 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 68 kDa.

Target

  • Relevance
    Protein phosphatase-1 (PP1) interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin cytoskeleton.
  • Cellular localization
    Cytoplasm; cytoskeleton
  • Database links
  • Alternative names
    • HKMT1098 antibody
    • KIAA1949 antibody
    • PPP1R18 antibody
    • Protein phosphatase 1 F actin cytoskeleton targeting subunit antibody
    • Protein phosphatase 1 regulatory subunit 18 antibody
    see all

Images

  • Anti-Phostensin antibody (ab103327) at 1/100 dilution + Ramos cell lysate at 35 µg

    Predicted band size : 68 kDa
  • ab103327, at 1/50 dilution, staining Phostensin in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References

ab103327 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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