Recombinant
RabMAb

Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

Overview

  • Product name
    Anti-PHOX2B antibody [EPR14423] - C-terminal
    See all PHOX2B primary antibodies
  • Description
    Rabbit monoclonal [EPR14423] to PHOX2B - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHOX2B aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99453

  • Positive control
    • Neuro-2a and SH-SY5Y cell lysates; Human neuroblastoma tissue; HeLa and SH-SY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/1000.
ICC/IF 1/100.

Target

  • Function
    Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
  • Tissue specificity
    Expressed in neuroblastoma, brain and adrenal gland.
  • Involvement in disease
    Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
    Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2) [MIM:613013]. A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.
  • Sequence similarities
    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NBLST2 antibody
    • NBPhox antibody
    • Neuroblastoma paired type homeobox protein antibody
    • Neuroblastoma Phox antibody
    • Paired like homeobox 2b antibody
    • Paired mesoderm homeobox 2b antibody
    • Paired mesoderm homeobox protein 2B antibody
    • Paired-like homeobox 2B antibody
    • PHOX 2B antibody
    • PHOX 2B homeodomain protein antibody
    • PHOX2B antibody
    • PHOX2B homeodomain protein antibody
    • PHX2B_HUMAN antibody
    • PMX 2B antibody
    • PMX2B antibody
    see all

Images

  • All lanes : Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) at 1/1000 dilution

    Lane 1 : Neuro-2a cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling PHOX2B  with ab183741 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

  • Immunofluorescence analysis of acetone-fixed HeLa cells labeling PHOX2B  with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

References

This product has been referenced in:
  • Olsen RR  et al. MYCN induces neuroblastoma in primary neural crest cells. Oncogene 36:5075-5082 (2017). IHC . Read more (PubMed: 28459463) »
  • Bouilloux F  et al. Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death. Elife 5:N/A (2016). Read more (PubMed: 26857994) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver bx - neuroblastoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
Permeabilization
No
Specification
Liver bx - neuroblastoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
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Submitted Dec 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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