Overview

  • Product name
  • Description
    Rabbit polyclonal to PHPT1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, from the N-terminal region of human PHPT1.

  • Positive control
    • PHPT1 transfected 293 cell lysate, human skeletal muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified through a protein A column, followed by immunogen affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 14, 60 kDa (predicted molecular weight: 14 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Relevance
    PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I. Protein histidine phosphorylation and dephosphorylation in eukaryotes has been sparsely studied compared to protein serine/threonine and tyrosine phosphorylation, although protein histidine phosphorylation is estimated to account for about 6% of total protein phosphorylation in eukaryotes. Ek et al. (2002) detected no activity of PHPT1 towards phosphoserine, phosphothreonine, or phosphotyrosine peptides.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • PHP14 antibody
    • 14 kDa phosphohistidine phosphatase antibody
    • CGI 202 antibody
    • HSPC141 antibody
    • Phosphohistidine phosphatase 1 antibody
    • PHPT 1 antibody
    • Protein janus A homolog antibody
    see all

Images

  • All lanes : Anti-PHPT1 antibody (ab71326) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : PHPT1 transfected 293 cell lysate

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Human skeletal muscle tissue with ab71326 at 1/10 dilution. DAB staining.

References

ab71326 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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