Overview

  • Product name
  • Description
    Rabbit polyclonal to PHRF1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1400 and 1450 of Human PHRF1 (NP_065952.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 179 kDa (predicted molecular weight: 179 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    PHRF1 contains 1 PHD-type zinc finger and 1 RING-type zinc finger. It may interact with POLR2A (via the C-terminal domain).
  • Database links
  • Alternative names
    • AA673488 antibody
    • CTD binding SR like protein rA9 antibody
    • KIAA1542 antibody
    • PHD and RING finger domain containing protein 1 antibody
    • PHD and ring finger domains 1 antibody
    • PHRF 1 antibody
    • RNF221 antibody
    see all

Images

  • All lanes : Anti-PHRF1 antibody (ab85974) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 179 kDa
    Observed band size : 179 kDa


    Exposure time : 10 seconds
  • Detection of PHRF1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85974 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:
  • Wang Y  et al. Overexpression of PHRF1 attenuates the proliferation and tumorigenicity of non-small cell lung cancer cells. Oncotarget 7:64360-64370 (2016). WB . Read more (PubMed: 27608840) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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