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Rabbit monoclonal [EPR13312] to PHYHD1
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHYHD1 aa 50-150. The exact sequence is proprietary.
Database link: Q5SRE7
- Human fetal liver and kidney lysates; HepG2 cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
||1/40 - 1/60.
Probably acts as alpha-ketoglutatare-dependent dioxygenase.
Belongs to the PhyH family. PHYHD1 subfamily.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- MGC16638 antibody
- OTTHUMP00000022302 antibody
- OTTHUMP00000022303 antibody
Western blot - Anti-PHYHD1 [EPR13312] antibody (ab181232)
All lanes :
Anti-PHYHD1 antibody [EPR13312] (ab181232) at 1/5000 dilutionLane 1 :
Human fetal kidney lysateLane 2 :
Human fetal liver lysateLane 3 :
HepG2 cell lysate
Lysates/proteins at 20 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) (ab136636
) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)Predicted band size:
Immunoprecipitation - Anti-PHYHD1 [EPR13312] antibody (ab181232)
Immunoprecipitation analysis of HeLa cells labeling PHYHD1 using ab181232 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at a 1/1000 dilution.
has not yet been referenced specifically in any publications.
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