The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1:62500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevancePHYHIP interacts with PHYH, suggesting a role in the development of the central nervous system. It may be involved in the development of neurological abnormalities observed in Down syndrome patients.