Anti-PI 3 Kinase catalytic subunit alpha antibody [4F3] (ab126819)

Overview

  • Product name
    Anti-PI 3 Kinase catalytic subunit alpha antibody [4F3]
    See all PI 3 Kinase catalytic subunit alpha primary antibodies
  • Description
    Mouse monoclonal [4F3] to PI 3 Kinase catalytic subunit alpha
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human PI 3 Kinase catalytic subunit alpha expressed in E. Coli.

  • Positive control
    • Recombinant Human PIK3CA protein (AA: 881-1068), HEK293 cell lysate and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab126819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 47.4, 124 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
  • Involvement in disease
    Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].
    Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance.
  • Sequence similarities
    Belongs to the PI3/PI4-kinase family.
    Contains 1 C2 domain.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
    • 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
    • caPI3K antibody
    • CLOVE antibody
    • CWS5 antibody
    • MCAP antibody
    • MCM antibody
    • MCMTC antibody
    • MGC142161 antibody
    • MGC142163 antibody
    • p110 alpha antibody
    • p110alpha antibody
    • Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody
    • Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic alpha polypeptide antibody
    • PI3 kinase p110 subunit alpha antibody
    • PI3-kinase subunit alpha antibody
    • PI3K antibody
    • PI3K-alpha antibody
    • PI3KC A antibody
    • PIK3C A antibody
    • Pik3ca antibody
    • PK3CA antibody
    • PK3CA_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns-3-kinase subunit alpha antibody
    • PtdIns-3-kinase subunit p110-alpha antibody
    • Serine/threonine protein kinase PIK3CA antibody
    see all

Images

  • Anti-PI 3 Kinase catalytic subunit alpha antibody [4F3] (ab126819) at 1/500 dilution + Recombinant Human PIK3CA protein (AA: 881-1068)

    Predicted band size : 47.4, 124 kDa
  • All lanes : Anti-PI 3 Kinase catalytic subunit alpha antibody [4F3] (ab126819) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 cell lysate


    Predicted band size : 47.4, 124 kDa
  • Example of standard curves obtained using ab126819. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red).
  • Immunofluorescence analysis of HeLa cells using ab126819 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye.
  • Flow cytometric analysis of Jurkat cells using ab126819 at 1/200 (green) and negative control (red).

References

ab126819 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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