Anti-PI 3 Kinase catalytic subunit alpha antibody (ab71090)
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Overview
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Product nameAnti-PI 3 Kinase catalytic subunit alpha antibody
See all PI 3 Kinase catalytic subunit alpha primary antibodies -
DescriptionRabbit polyclonal to PI 3 Kinase catalytic subunit alpha
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide (Human) from the the center region of PI 3 Kinase catalytic subunit alpha conjugated to KLH
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Positive control
- WB: K562 cell lysate and 293 cell line lysates transiently transfected with the PI 3 Kinase catalytic subunit alpha gene. IHC-P: Human breast carcinoma tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab71090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | 1/50 - 1/100. Predicted molecular weight: 124 kDa. | |
| ELISA | 1/1000. | |
| IHC-P | 1/50 - 1/100. |
Target
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FunctionPhosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
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Involvement in diseaseDefects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].
Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance. -
Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 C2 domain.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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Database links
- Entrez Gene: 5290 Human
- Omim: 171834 Human
- SwissProt: P42336 Human
- Unigene: 553498 Human
- Unigene: 715194 Human
- Unigene: 85701 Human
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Alternative names
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
- 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
- caPI3K antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha antibody (ab71090)ICC/IF image of ab71090 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71090 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - PI 3 Kinase catalytic subunit alpha antibody (ab71090)Anti-PI 3 Kinase catalytic subunit alpha antibody (ab71090) at 1/60 dilution + K562 cell lysate at 35 µg
Predicted band size: 124 kDa
Observed band size: 130 kDa (why is the actual band size different from the predicted?)
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Western blot - PI 3 Kinase catalytic subunit alpha antibody (ab71090)All lanes : Anti-PI 3 Kinase catalytic subunit alpha antibody (ab71090) at 1/60 dilution
Lane 1 : Non transfected 293 cell lysate
Lane 2 : 293 cell lysate transiently transfected with the PI 3 Kinase catalytic subunit alpha gene
Lysates/proteins at 1 µg per lane.
Predicted band size: 124 kDa
Observed band size: 114 kDa (why is the actual band size different from the predicted?)
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PI 3 Kinase catalytic subunit alpha antibody (ab71090)Formalin fixed and paraffin embedded human breast carcinoma tissue labelled with ab71090 at a 1/50 dilution. ab71090 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
Protocols
References
ab71090 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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