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Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma
Skeletal muscle tissue, 293 cell lysates transiently transfected with the PI 3 Kinase catalytic subunit (PI3KCG) gene.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Protein A purified
This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
1/10 - 1/50.
3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
Pancreas, skeletal muscle, liver and heart.
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
Information by UniProt
1 phosphatidylinositol 3 kinase antibody
5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
Western blot - PI 3 Kinase catalytic subunit gamma antibody (ab70903)
All lanes : Anti-PI 3 Kinase catalytic subunit gamma antibody (ab70903) at 1/100 dilution Lane 1 : Non transfected 293 cell lysate Lane 2 : PI 3 Kinase catalytic subunit transfected 293 cell lysate Lysates/proteins at 2 µg per lane. Predicted band size : 126 kDa Observed band size : 126 kDa Additional bands at : 140 kDa,55 kDa,72 kDa,90 kDa,95 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PI 3 Kinase catalytic subunit gamma antibody (ab70903)
Formalin-fixed and paraffin-embedded skeletal muscle tissue labelled with ab70903 at 1/10 dilution followed by peroxidase conjugated secondary antibody step and DAB staining.
has not yet been referenced specifically in any publications.
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